Spectrophotometer

Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. Light from a white light source is split into its components by a monochromator such as a diffraction grating or a prism and directed to an exit slit. Beyond the slit, the selected light color passes through a cuvette holding the test sample and falls on a photo-receptor that allows calculating the absorbance via the Lambert-Beer equation A = -Log₁₀(I₁/I₀).

Note: The first version of the spectrophotometer was made by Ashish and Ravi with help from GN and I. Shirish, Praveen and Ankush provided valuable advice.