Sunday, December 30, 2018

Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. Light from a white light source is split into its components by a monochromator such as a diffraction grating or a prism and directed to an exit slit. Beyond the slit, the selected light color passes through a cuvette holding the test sample and falls on a photo-receptor that allows calculating the absorbance via the Lambert-Beer equation A = -Log10(I1/I0).

Note: The first version of the spectrophotometer was made by Ashish and Ravi with help from GN and I. Shirish, Praveen and Ankush provided valuable advice.

side views with stepper motor control buttons
view from top, front-to-back and back-to-front